Immediate graft histological assay, post pig's liver transplantation.

PURPOSE: To describe the vascular and tissue histopathological changes in seven sequential experimental liver transplantations in pigs. METHODS: Fourteen female pigs, Sus domesticus species, with body mass between 5 and 8 kg were utilized. After the end of all anastomoses of the graft implantation in the receptor, the animal was monitored for 30 minutes, and at its end one of the biopsies was collected for histological analysis. The histological criteria utilized were: lytic hepatocyte necrosis, density of septal and portal inflammatory infiltrated, sinusoidal congestion and hemorrhage. The analysis was performed separately for the portal region in zone 1, 2 and 3. RESULTS: Among the structural changes undergone by the graft, those with greater frequency and intensity were vascular congestion and steatosis, which stood out in transplantations 5, 6 and 7. CONCLUSIONS: The technique demonstrated vascular alterations represented by vasocongestion, edema and minimum inflammatory reaction. In relation to the parenchyma, was observed macrovacuolar pan-acinar steatosis, focal lytic and occasional hemorrhages, beyond the accumulation of hemosiderin in Kuppfer's cells.

Immediate graft histological assay, post pig's liver transplantation

PURPOSE: To describe the vascular and tissue histopathological changes in seven sequential experimental liver transplantations in pigs. METHODS: Fourteen female pigs, Sus domesticus species, with body mass between 5 and 8 kg were utilized. After the end of all anastomoses of the graft implantation in the receptor, the animal was monitored for 30 minutes, and at its end one of the biopsies was collected for histological analysis. The histological criteria utilized were: lytic hepatocyte necrosis, density of septal and portal inflammatory infiltrated, sinusoidal congestion and hemorrhage. The analysis was performed separately for the portal region in zone 1, 2 and 3. RESULTS: Among the structural changes undergone by the graft, those with greater frequency and intensity were vascular congestion and steatosis, which stood out in transplantations 5, 6 and 7. CONCLUSIONS: The technique demonstrated vascular alterations represented by vasocongestion, edema and minimum inflammatory reaction. In relation to the parenchyma, was observed macrovacuolar pan-acinar steatosis, focal lytic and occasional hemorrhages, beyond the accumulation of hemosiderin in Kuppfer's cells.

Anesthetic technique for liver transplantation in pigs / Técnica anestésica no transplante de fígado em suínos

Introduction: the interventionists procedures, as in the case of liver surgeries, needs anesthesia toprovide ideal conditions for the patient. Among the animals utilized for experimentation, pigs arehighlighted by their anatomical similarity to humans, being, therefore, a good animal to be studied.Objective: realize anesthesia in surgical procedures on medium-sized animals, mainly in pigs, dueto easy performance and low cost. Method: for this study, fourteen pigs weighing between 8-12 kgwere utilized. Sedation with the application of ketamine was carried out (10 mg/kg) associated withxylazine (1 mg/kg). Then, a catheterization of the internal jugular vein and internal carotid arterywas realized. After this procedure, it was initiated the maintenance with the administration offentanyl 3-5 ?g/kg and ketamine 2-5mg/kg and followed this time with the installation of halothaneand oxygen. Conclusion: the anesthetic technique proposed was easily carried out by trainedprofessionals or students, enabling the realization of liver transplantations as well as other surgeries,demonstrating to be safety for the manipulator and the animal.

Introdução: os procedimentos que têm de intervenção, como no caso das cirurgias de fígado,necessita de anestesia para manter o paciente em condições ideais. Entre os animais utilizados nasexperiências, os porcos são destacados por sua similaridade anatômica para os seres humanos,sendo, portanto, um bom animal a ser estudado. Objetivo: a realização de anestesia emprocedimentos em animais de médio porte, principalmente em suínos por desempenho fácil e debaixo custo. Método: para este estudo, 14 porcos com peso entre 8-12 kg foram utilizados. Sedaçãocom a aplicação de cetamina foi realizada (10 mg / kg) associada com xilazina (1 mg / kg). Emseguida, um cateterismo de veia jugular interna e artéria carótida interna foi realizado. Após esteprocedimento, iniciou-se a manutenção com a administração de fentanil 2-5mg/kg 3-5 mg / kg ecetamina e seguiu-se desta vez com a instalação de halotano e oxigênio. Conclusão: a técnicaanestésica proposta foi facilmente realizada por profissionais treinados ou estudantes, permitindo arealização de transplantes de fígado, assim como outras cirurgias, demonstrando ser de segurançapara o manipulador e o animal.

Immediate intra and post-surgery complications of the orthotopic liver transplantation in pigs

Objective: to analyze immediate organic modifications and complications subsequent to orthotopic liver transplantation in pigs. Method: fourteen female pigs, specie Sus domesticus, were utilized in this study, with body mass between 5 and 8 Kg, considering that we evaluated the main immediate intra and post-surgery complications of the liver transplantation. Results: in the donor, we highlighted as the main complication the occurrence of bleedings, considering that this was observed in all procedures. In addition, such situation occurred during surgery involving the receptor, in which the bleeding was also perceived as the most frequent complication. However, it is valid to highlight that the hemodynamic instability was present in 5 procedures involving the receptor. Conclusion: among trans surgery complications we may cite: difficulty of oro-tracheal intubation, intra surgery hemorrhages, hemodynamic and respiratory instability and anesthetic complications. In the immediate post-surgery, thus, we observed as the most frequent complications: hemodynamic instability and small anastomotic bleedings

Objetivo: analisar as modificações orgânicas e complicações imediatas subseqüentes ao transplante hepático ortotópico em suínos. Método: foram utilizados no estudo, 14 porcos fêmeas espécie Sus domesticus, com massa corporal entre 5 e 8 Kg, sendo que avaliou-se as principais complicações intra e pós-operatórias imediatas no transplante hepático. Resultados: no doador, destacou-se a ocorrência de sangramentos como principal complicação, tendo em vista que esta foi observada em todos os procedimentos. Tal situação também ocorreu no ato cirúrgico envolvendo o receptor, no qual se percebeu o sangramento como a complicação, também, mais freqüente. Entretanto é valido destacar a instabilidade hemodinâmica, que esteve presente em 5 procedimentos envolvendo o receptor. Conclusão: dentre as complicações trans operatórias podem ser citadas: dificuldade de intubação oro-traqueal, hemorragias intra operatórias, instabilidade hemodinâmica e respiratória e complicações anestésicas. Já no pós-operatório imediato observamos como complicações mais frequentes: instabilidade hemodinâmica e pequenos sangramentos anastomóticos.